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Shared Facilities

Confocal Microscopy


Multiphoton Confocal Fluorescence Microscopy is a powerful research tool that combines the advanced optical techniques of laser scanning microscopy with long wavelength multiphoton fluorescence excitation to capture high-resolution, three dimensional images of specimens tagged with highly specific fluorophores. National Cancer Centre Singapore has one such Multi-Photon Confocal Fluorescent Microscope (MCRM) available if users require the imaging of tissues or other thick scattering speciments in three-dimension.

Flow Cytometry

The Flow Cytometry facility is equipped with both the BD FACSCaliburTM system and the FACSCanto II Flow Cytometry system. The BD FACSCanto II Flow Cytometry system is the flexible, modular design which offers automation and up to eight-colour capacity for rapid and easy production of high-quality data, while the FACSCalibur is the only four-colour, dual-laser, benchtop system available that is capable of both analysis and sorting.

Centrifuge Room

We provide ultra high speed centrifuges, high speed refrigerated centrifuges and a speedvac concentrator in the shared centrifuge facility. The facility is also equipped with a cyrostat to cut histological slides.

Irradiator Room

We have a Caesium-137 self contained irradiator facility, which is used to irradiate cell lines and small animals. It is made available to investigators at the National Cancer Centre Singapore and users are required to have a R1 license from NEA. Users are mandated to wear their TLD badges while operating the irradiator.

Histology

Within NCCS, equipment is available to produce quality microscopic sections of frozen and parraffin embedded material. We have Automated Tissue Processors, a Tissue Embedding Station, microtomes, cryostats, along with image capture and image analysis. In addition to light microscopes, NCCS has a confocal microscope system with image analysis (refer to Confocal Microscopy).

Dark Room

A tabletop Film Processor, designed for medium- and low- volume processing applications is available.